Lead Researcher in Blue Sky Protein Kinases

Company: St. Jude Children's Research Hospital
Location: Memphis
Posted on: February 18, 2025

Job Description:

We are looking for an experienced biochemist to participate in our exciting studies of the human protein kinome.As part of the largest BlueSky initiative, "Seeing the Invisible in Protein Kinases" at St. Jude Children's Research Hospital, we are expanding our structural studies of the human kinome to identify novel kinase-binding ligands. We are looking for an experienced biochemist to aid in these efforts.The candidate will work in a fast-paced research environment, studying protein kinases by generating and characterizing high quality proteins, assessing binding affinity and kinetics using various biophysical techniques in collaboration with Nuclear Magnetic Resonance (NMR) spectroscopists and computational biologists. This role will involve working closely as part of a multidisciplinary team including computational chemists, structural biologists, biochemists, and other biophysical specialists. The unparalleled NMR center at St. Jude includes three 600 MHz, two 700 MHz, six 800 MHz, 850 MHz, and the first 1.1 GHz in the world.Responsibilities:

• Perform tasks related to the research project independently, but within specific guidelines and subject to regular review by supervisor or other research staff.
• Perform basic molecular biology techniques like polymerase chain reaction (PCR), vector construction, plasmid purification and sequencing analysis.
• Responsible for expression, purification, modification, and characterization of recombinant proteins from a variety of host cells.
• Design and implement purification schemes and protein analytics, present results, and interpretation.
• Lead troubleshooting efforts for protein expression and purification processes.
• Work effectively in a collaborative team environment where results are achieved through influence, cooperation, and the incorporation of multiple points of view.Ideal Candidate:
• Proven expertise in advanced molecular cloning techniques.
• Experience generating chimeric recombinant sequences, site-directed mutagenesis, random mutagenesis, codon optimization, and/or gene synthesis is highly desired.
• Strong background in protein biochemistry, protein purification (using affinity and traditional chromatographic techniques), and biophysical characterization.
• Hands-on experience with instrumentation essential for protein purification and characterization, especially BioRad/AKTA FPLC, SDS PAGE and Western Blot.
• Work independently to perform protein analysis including (but not limited to) CE SDS, ITC, SPR, U/HPLC, DSF, and cell based functional assays.
• Excellent oral, presentation and written communication skills required.Minimum Education and/or Training:Bachelor's degree in a relevant scientific area is required.Minimum Experience:5+ years of research experience is required with a Bachelor's degree.

• 3+ years of experience is required with a Master's degree.
• No prior experience required with a PhD.
• Strong knowledge and experience in understanding and application of underlying principles related to techniques being performed.
• Experience in R&D methods and processes in lab setting, and execution of research projects for the laboratory preferred.
• Proven performance in earlier role/comparable role.St. Jude Children's Research Hospital offers a remarkable opportunity to perform cutting edge research with outstanding institutional support and exceptional shared core facilities in an environment of open collaboration, educational advancement, and training opportunities. St. Jude is consistently ranked on Fortune Magazine's and Glassdoor's "100 Best Places to Work" for companies with 1,000 or more employees. We are located in the heart of Memphis, Tennessee, a vibrant and friendly city at the historic American crossroads of music, trade, food, and culture. Mild winters and beautiful green outdoor spaces are part of the reasons why Forbes recently named Memphis the 4th happiest city in which to work. Cost of living and high quality of life are other attractions: Money magazine listed Memphis as one of the least expensive cities in which to raise a child. St. Jude facilities and salaries are highly competitive.CompensationIn recognition of certain U.S. state and municipal pay transparency laws, St. Jude is including a reasonable estimate of the compensation range for this role. This is an estimate offered in good faith and a specific salary offer takes into account factors that are considered in making compensation decisions including but not limited to skill sets, experience and training, licensure and certifications, and other business and organizational needs. It is not typical for an individual to be hired at or near the top of the salary range and compensation decisions are dependent on the facts and circumstances of each case. A reasonable estimate of the current salary range is $60,320 - $105,040 per year for the role of Lead Researcher in Blue Sky Protein Kinases.Explore our exceptional !Diversity, Equity and InclusionSt. Jude Children's Research Hospital has a diverse, global patient population and workforce, built on the principles of diversity, equity and inclusion. Our founder Danny Thomas envisioned a hospital that would treat children of the world-regardless of race, religion or a family's ability to pay. .Today, we continue the mission to advance cures and means of prevention for pediatric catastrophic diseases through research and treatment. As we accelerate this progress globally, we believe our legacy of diversity, equity and inclusion is foundational to success. With the commitment of leaders at all levels of the organization, we strive to ensure the St. Jude culture, leadership approaches and talent processes are equitable and culturally responsive. View our to learn about the hospital's roots in diversity, equity and inclusion, where we are today and our aspirations for an even better future.No Search FirmsSt. Jude Children's Research Hospital does not accept unsolicited assistance from search firms for employment opportunities. Please do not call or email. All resumes submitted by search firms to any employee or other representative at St. Jude via email, the internet or in any form and/or method without a valid written search agreement in place and approved by HR will result in no fee being paid in the event the candidate is hired by St. Jude.
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Keywords: St. Jude Children's Research Hospital, Memphis , Lead Researcher in Blue Sky Protein Kinases, Other , Memphis, Tennessee